Long-Wavelength Calcium Indicators
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Innovative Methodology Presynaptic Calcium Measurements at Physiological Temperatures Using a New Class of Dextran-Conjugated Indicators
Beierlein, Michael, Kyle R. Gee, Vladimir V. Martin, and Wade G. Regehr. Presynaptic calcium measurements at physiological temperatures using a new class of dextran-conjugated indicators. J Neurophysiol 92: 591–599, 2004; 10.1152/jn.00057.2004. Presynaptic calcium (Capre) has been studied extensively because of its role in triggering and modulating neurotransmitter release. Although calcium reg...
متن کاملPresynaptic calcium measurements at physiological temperatures using a new class of dextran-conjugated indicators.
Presynaptic calcium (Ca(pre)) has been studied extensively because of its role in triggering and modulating neurotransmitter release. Although calcium regulation and calcium-driven processes can be strongly temperature dependent, technical difficulties have limited most studies of Ca(pre) to temperatures well below the physiological range. Here we assessed the use of membrane-permeant acetoxyme...
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The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in viv...
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The introduction of calcium ion (Ca(2+)) indicators based on red fluorescent proteins (RFPs) has created new opportunities for multicolour visualization of intracellular Ca(2+) dynamics. However, one drawback of these indicators is that they have optimal two-photon excitation outside the near-infrared window (650-1,000 nm) where tissue is most transparent to light. To address this shortcoming, ...
متن کاملTopical application of indicators for calcium imaging at the Drosophila larval neuromuscular junction.
Calcium imaging is a technique in which Ca(2+)-binding molecules are loaded into live cells and as they bind Ca(2+) they "indicate" the concentration of free calcium through a change in either the intensity or the wavelength of light emitted (fluorescence or bioluminescence). There are several possible methods for loading synthetic Ca(2+) indicators into subcellular compartments, including topi...
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